鼠疫免疫学检测技术的应用和研究进展
Progress in the application and study of immunological detection technology for Yersinia pestis

鼠疫免疫学检测技术是掌握鼠疫流行动态,发现新鼠疫疫源地,确诊疑似鼠疫患者的重要手段。目前,鼠疫常用的免疫学检测方法有间接血凝试验(IHA)、酶联免疫吸附试验(ELISA)、放射免疫沉淀试验(RIPA)、胶体金免疫层析技术(GICA)以及酶免疫染色技术(EIT)等,各种检测技术皆有其优缺点,IHA和ELISA主要用于鼠疫检测;GICA和EIT主要用于人间鼠疫疫情处置工作中;RIPA因存在对人有害的放射性问题,现已不再使用。在鼠疫防控实际工作中,多种检测技术联合应用可避免假阳性,能起到更好的效果。

Immunological detection technology for Yersinia pestis is an important tool to master the epidemic dynamics of plague, discover new plague foci, and confirm suspected plague cases. Now, the commonly used immunological detection methods for Y. pestis include indirect hemagglutination assay(IHA), enzyme-linked immunosorbent assay(ELISA),radioimmunoprecipitation assay(RIPA), colloidal gold immunochromatography assay(GICA), and enzyme immunostaining technique(EIT). Each detection technology has its advantages and disadvantages. IHA and ELISA are mainly used for plague detection. GICA and EIT are used for human plague epidemics. RIPA is no longer used due to its radioactive harm to humans. In the actual plague prevention and control, a combination of multiple detection technologies, which could avoid false-positives and achieve better results, would be a better choice.

国家科技重大专项(2018ZX10101002-002-009)~~;

鼠疫; 免疫学; 检测技术;

Yersinia pestis; Immunology; Detection technology;

R516.8;R446.6

202228-2314914K